Kosheeka : Primary Cells for Research

  Human skin acts as the first line of the body's defense, that constantly encounters mechanical damage, environmental stress, or exposure to chemicals. Human dermal fibroblasts are metabolically active, dynamic cellular networks found underneath the epidermis. They mainly promote: - the production of extracellular matrix (ECM),  - preservation of structural integrity, and  - wound healing.  Accordingly, with increasing interest in regenerative medicine, tissue engineering, drug screening, and cosmetic science, these fibroblasts have emerged as key tools in research laboratories worldwide. Biological Importance of Human Dermal Fibroblasts Human Dermal Fibroblasts distribute uniformly in the dermis and spatially in a dispersed manner. Moreover, these subpopulations are morphologically and functionally specific, and different in terms of gene expression patterns: - The reticular fibroblasts form the dense collagen network that makes the skin form tensile strength....

  The field of experimental biology is rapidly developing, and researchers require models precisely resembling human tissues, disease mechanisms, and responses to therapeutic methods. This has further necessitated quality biomedical research cells, such as primary cells, engineered lines, iPSC derivatives, and 3D constructs in academic, clinical, and industrial laboratories.  Biomedical Research Cells: The Foundation of Translational Assessments These cells are useful because they can be used as regulated in vitro systems to decipher cellular physiology, gene regulatory networks, and functional outputs: 1. Better Fidelity to Human Physiology Compared with conventional animal models, human primary cells and stem cell-based models enable researchers to circumvent interspecies variation and obtain more predictive modeling. 2. Compatibility with Advanced Technologies The next-generation tools that Biomedical Research Cells can be integrated with include: Single-cell transcriptomi...

  The goal of every drug discovery is to develop safe and effective medications. To achieve this crucial goal, a key step involves studying the metabolism of drugs in order to understand their potential toxicity and effectiveness. Even before we start preclinical trials on human primary cells or animals, we need to test the drugs in vitro to ensure their safety and efficacy. One major step forward is the use of mouse liver subcellular fractions. Why are these fractions important? New drugs need to be safe and effective. Part of this safety check involves understanding how our bodies metabolize the drug. These mouse hepatocyte S9 fractions mimic the human liver's metabolic machinery, helping researchers predict how a drug will be processed in the body. We have three types of fractions, namely S9, microsome, and cytosol, that can offer a significant understanding to improve drug discovery. Think of these fractions as specialized teams within the liver cell, each with a specific role ...

               The tiny powerhouses within us—CELLS—play a starring role in the exciting world of drug discovery! But how do researchers utilize different cell types to develop new treatments? It all starts with the cell selection dilemma! Wait, let me explain what I meant! Consider our body as a vast library, but instead of books, it's filled with millions of cell types, each with unique functions. If you have to pick one book and try to alleviate what is plaguing it, it will be challenging. That is the same dilemma researchers face: choosing the right "cell-ebrity" for their specific research needs. There are three main categories of the cells that are present: Immortalized Cell Lines: These are the cells that can be grown indefinitely, offering a reliable source for various experiments. However, they do not always perfectly reflect what happens inside the body. Dissociated Tumor Cells (DTCs): Directly extracted from tumors, these cells offer ...

  When it comes to understanding cancer, it is crucial to recognize the various types and their distinct characteristics. Two major categories of cancer that often arise in medical discussions are sarcoma and carcinoma. While both originate in different types of tissues, their progression, treatment options, and prognosis can significantly vary. In this blog, we will explore the origins of sarcoma and carcinoma and their unique progression patterns, and discuss how these differences impact their treatment approaches. Understanding Sarcoma Sarcoma is a type of cancer that develops from connective tissues, including bones, muscles, cartilage, blood vessels, and soft tissues. Unlike carcinoma, which typically originates from epithelial cells, sarcomas arise from mesenchymal cells . This distinction in tissue origin gives rise to notable differences in how sarcomas progress and spread. Origin and Progression of Sarcoma Sarcomas often begin in the supporting structures of the body, such...

  Animal tissue culture, also known as animal cell culture, is a remarkable scientific technique that involves growing and maintaining animal cells in an artificial environment. In the realm of scientific research, animal tissue culture stands as a powerful and versatile technique that has revolutionized multiple fields. This method has revolutionized various areas of research, offering new insights into biology, medicine, veterinary science, and biotechnology. In this blog, we will delve into the fascinating world of animal tissue culture, explore its applications, and understand its significance in advancing scientific knowledge. What is Animal Tissue Culture? Animal tissue culture involves the isolation and cultivation of animal cells in an artificial environment. Cells can be derived from diverse sources, including organs, tissues, or embryos. Once isolated, these cells are placed in a suitable nutrient medium that provides them with essential nutrients, growth factors, and opt...

  The human body is composed of various tissues that work together to maintain its structure and function. While some tissues, such as bones and muscles, have the ability to regenerate naturally, others may require additional support through nutrition. Here are six bodily tissues that can be regenerated through proper nutrition: Skin Tissue: The skin is the body's first line of defence against environmental factors like pollution, UV radiation, and toxins. It is constantly being exposed to these harmful substances, which can damage the skin's cells and cause premature ageing . However, by consuming certain nutrients, we can support the growth and repair of skin tissue . Vitamin A, for example, is essential for maintaining healthy skin and mucous membranes. It helps to regulate skin cell growth and differentiation, which is important for wound healing and preventing skin damage. Vitamin C is another important nutrient for skin health, as it plays a role in collagen production. ...

The skin is one of the largest and most complex organs in the human body, and its function as a barrier to the external environment is critical for maintaining overall health and wellness. A key component of the skin is the dermis, a layer of tissue located beneath the epidermis that provides structural support and plays a critical role in wound healing and tissue repair. Dermal cells, specifically dermal fibroblasts and dermal macrophages are key components of the dermis and play important roles in maintaining skin health and function. Dermal fibroblasts are cells responsible for the production of extracellular matrix components such as collagen, elastin, and glycosaminoglycans. These components provide the structural support that gives the skin its strength and elasticity. Additionally, dermal fibroblasts play an important role in wound healing and tissue repair. Upon injury, dermal fibroblasts respond by proliferating and producing matrix components that help to close the wound and...

The cluster of differentiation methodology used to identify cell surface antigens is where CD34 gets its name. Civin et al. and Tindle et al. simultaneously described CD34 for the first time on hematopoietic stem cells as a cell surface glycoprotein that serves as a cell-cell adhesion factor. Additionally, it might facilitate the bonding of hematopoietic stem cells with stromal cells or the extracellular matrix of bone marrow. In terms of medicine, it relates to the choice and enhancement of hematopoietic stem cells for bone marrow transplants. Although CD34 expression is actually seen on many other cell types, it is typically always associated with hematopoietic cells because of these historical and clinical links. Functions of CD 34 The CD34 protein is a member of a family of single-pass transmembrane sialomucin proteins that show the expression on early haematopoietic and vascular-associated progenitor cells. However, little is known about its exact function. CD34 is also an import...