Kosheeka : Primary Cells for Research

A major health issue impacting 9.1% of the world's population is chronic kidney disease (CKD). However, there are still few options for CKD progression prevention. Renal fibrosis, renal anaemia, and peritubular capillary loss are three clinical diseases associated with CKD that are significantly influenced by resident human kidney fibroblast , a type of primary cells . By manufacturing extracellular matrix proteins and erythropoietin under healthy conditions, kidney fibroblasts support the structure of the organ. Numerous studies have shown that fibroblasts play advantageous functions in the regeneration of renal tubules. In some clinical situations, renal fibroblasts have the capacity to develop into a proinflammatory state, produce a variety of cytokines and chemokines, and extend inflammation by creating tertiary lymphoid tissues, functioning lymphoid aggregates. In this article, we discuss the various roles that renal fibroblasts play in both healthy and pathological states. Fu...

  The coronavirus disease-19 (COVID-19) pandemic has caused the world unspeakable harm. Given how quickly the pandemic has spread, it is crucial to think of a variety of treatment alternatives in order to successfully treat people worldwide. Since the immune system is the focal point of the infection, it is crucial to control the dynamic equilibrium in order to avoid exaggerated immunological responses that ultimately cause harm to several organs.  In the last ten years, there has been a significant increase in the use of stem cells for medical purposes. Mesenchymal stem cells (MSCs) and exosomes isolated from MSCs (MSC-Exo) have been made available to the world as potential therapeutics for a wide range of ailments as a result of scientifically ground-breaking methods. Patients with COVID-19 may benefit from using MSCs and MSC-Exos as a therapy to regulate their immune systems because they have immunomodulatory properties.  MSC-Exosomes and Immunomodulation It is now r...

Animal use is required for preclinical investigations in life science research, which is time-consuming, costly, and causes animal cruelty. Animals being used in scientific research and tests has drawn criticism from environmentalists and animal rights activists. Several laws have also been proposed to restrict the use of animals in research. Researchers have been compelled by this to come up with alternatives that will promote compassion for animals while simultaneously reducing the number of animals exploited. In silico computer simulation and in vitro cell cultures are the two main substitutes for in vivo animal experimentation. Cell lines are now a simple tool to study nearly every subject connected to health and disease because of developments in cultural techniques. The ability to easily cultivate cells on dishes makes cell lines an effective tool for experimental studies. Cells can be easily cultivated in small containers at the workstation, and the number of cells can be simpl...

  Cancer researchers can improve and hone cell and gene therapy choices to provide individualised medical treatments through the immunomodulatory properties of mesenchymal stem cells. Cancer medicine and therapy developers can alter the functioning of the cells to promote cancer suppression by utilising mesenchymal stem cells' capacity to modify the tumour microenvironment. Mesenchymal stem cells , for instance, present a route to deliver anti-cancer medications to tumour locations due to their propensity to move to tumours. Additionally, researchers can control mesenchymal stem cells via antibody treatment and modify the angiogenesis process, which cancers depend on to thrive. Immunomodulatory effects of MSCs The immunomodulatory characteristics of MSCs offer researchers a powerful weapon against cancer, serving as the foundation for immunotherapy . The development of immune system cells such macrophages, dendritic cells, T cells, B cells, and natural killer cells (NK cells), whi...

The reproducibility and validity of scientific findings are shaped by a multitude of factors, and precise experimental methodologies, as well as the use of identical experimental substances where applicable, must be recorded. Reproducibility necessitates the characterization of reagents to assure their purity. Cell culture is an essential component of current biomedical research. Multiple quality control techniques, including cell line identification and validation that cell lines are not contaminated, are essential to assuring that reported data is reproducible. Cell line authentication is required to guarantee that cell lines have not been unintentionally incorrectly labelled or cross-contaminated, which could contribute to incorrect disease model interpretations. The necessity of quality control of cell lines used in scientific research cannot be overstated, as it is vital for data reproducibility. Two major hazards in tissue culture are: 1.        C...

According to The European Review for Medical and Pharmacological Sciences (2016), cells derived from the mesoderm with the ability to undergo self-renewal are mesenchymal stem cells (MSCs). These cells were first described by a team led by Friedenstein as spindle-shaped cells that adhered to the culture vessel from the bone marrow. MSCs can differentiate into several cell types such as chondrocytes (cartilage cells), osteocytes (bone cells), adipocytes (fat cells) and myocytes (muscle). 2016-published research in the Journal of Orthopaedic Surgery and Research by scientists Viganò and team explained that these cells maintain the optimum number of cells in tissues to maintain tissue homeostasis by differentiating into appropriate cells. They also release many growth factors and molecules that regulate the immune system. A team led by Sriramulu (2018) described added features of these cells in the International Journal of hematology-oncology and stem cell rese...