Kosheeka : Primary Cells for Research

  Primary cells have also been used in life sciences research, and there is a high demand for these cells. The main advantages of primary cells over immortalised cells are their functional and genetic adherence. Primary cell information is comparable to physiologic significance because these cells lack the biological modifications that enable indefinite in vitro cultivation. However, primary cells pose difficulties. Their supply is constrained, they are difficult to obtain and isolate, they are intolerant of all but the most specific culture conditions, and they don't live long: Primary cells have a maximum of 15 to 20 passages, whereas immortalised cell lines live indefinitely. These issues, however, are usually manageable with meticulous optimization of  culture methods, media, and other factors. Disease-Specific Primary Cells Primary cells require unique environmental and nutritional conditions due to their high level of specialisation in order to grow and maintain the pro...

The reproducibility and validity of scientific findings are shaped by a multitude of factors, and precise experimental methodologies, as well as the use of identical experimental substances where applicable, must be recorded. Reproducibility necessitates the characterization of reagents to assure their purity. Cell culture is an essential component of current biomedical research. Multiple quality control techniques, including cell line identification and validation that cell lines are not contaminated, are essential to assuring that reported data is reproducible. Cell line authentication is required to guarantee that cell lines have not been unintentionally incorrectly labelled or cross-contaminated, which could contribute to incorrect disease model interpretations. The necessity of quality control of cell lines used in scientific research cannot be overstated, as it is vital for data reproducibility. Two major hazards in tissue culture are: 1.        C...

According to The European Review for Medical and Pharmacological Sciences (2016), cells derived from the mesoderm with the ability to undergo self-renewal are mesenchymal stem cells (MSCs). These cells were first described by a team led by Friedenstein as spindle-shaped cells that adhered to the culture vessel from the bone marrow. MSCs can differentiate into several cell types such as chondrocytes (cartilage cells), osteocytes (bone cells), adipocytes (fat cells) and myocytes (muscle). 2016-published research in the Journal of Orthopaedic Surgery and Research by scientists Viganò and team explained that these cells maintain the optimum number of cells in tissues to maintain tissue homeostasis by differentiating into appropriate cells. They also release many growth factors and molecules that regulate the immune system. A team led by Sriramulu (2018) described added features of these cells in the International Journal of hematology-oncology and stem cell rese...

Consistency in research experiments is hard to achieve. There are several factors that can affect data reproducibility. Publishing data in high impact journals requires repeating experiments and standardization of techniques and procedures. We summarize 4 major factors for generating robust and reliable data. Reagents As a rule, the brand and catalog numbers of products for a specific experiment or project must be fixed to ensure low variability in the data generated. One must use reagents from reputed brands with citations to maintain credibility of the results. Verifying the quality of the reagents will save both time and effort involved in planning and execution. This can be done by a thorough literature study and checking user reviews before ordering the product. If working with cell culture , the date of opening of cell culture media and its expiry must be labeled and checked before using it. This also applies to other reagents such as FBS, trypsin or PBS, etc. Old reagents ...