Kosheeka : Primary Cells for Research

The reproducibility and validity of scientific findings are shaped by a multitude of factors, and precise experimental methodologies, as well as the use of identical experimental substances where applicable, must be recorded. Reproducibility necessitates the characterization of reagents to assure their purity. Cell culture is an essential component of current biomedical research. Multiple quality control techniques, including cell line identification and validation that cell lines are not contaminated, are essential to assuring that reported data is reproducible. Cell line authentication is required to guarantee that cell lines have not been unintentionally incorrectly labelled or cross-contaminated, which could contribute to incorrect disease model interpretations. The necessity of quality control of cell lines used in scientific research cannot be overstated, as it is vital for data reproducibility. Two major hazards in tissue culture are: 1.        C...

According to The European Review for Medical and Pharmacological Sciences (2016), cells derived from the mesoderm with the ability to undergo self-renewal are mesenchymal stem cells (MSCs). These cells were first described by a team led by Friedenstein as spindle-shaped cells that adhered to the culture vessel from the bone marrow. MSCs can differentiate into several cell types such as chondrocytes (cartilage cells), osteocytes (bone cells), adipocytes (fat cells) and myocytes (muscle). 2016-published research in the Journal of Orthopaedic Surgery and Research by scientists Viganò and team explained that these cells maintain the optimum number of cells in tissues to maintain tissue homeostasis by differentiating into appropriate cells. They also release many growth factors and molecules that regulate the immune system. A team led by Sriramulu (2018) described added features of these cells in the International Journal of hematology-oncology and stem cell rese...