Kosheeka : Primary Cells for Research

  Ageing is an inescapable process that causes a steady functional impairment in the large majority of organisms. Changes in the looks of skin, which becomes thin, less supple, wrinkled, and far less able to accomplish its defensive role, are one of the most visible indicators of ageing in people. Skin ageing is a multi-faceted phenomenon, and experts are still trying to figure out all of the intricate connections that occur. We do know, however, that it is influenced by both inherent (intrinsic) and external (extrinsic) variables. Intrinsic wrinkles are characterized by skin shrinkage, an enhanced predominance of the vasculature, decreased elasticity, and fine wrinkles, and are mostly genetically driven. Extrinsic causes comprise Exposure to UV radiation and pollution, which cause intense wrinkles, rough texture, and pigmentation anomalies. A mismatch of pro-and anti-oxidants causes oxidative stress, which promotes quicker skin ageing, inflammation, and disease. Currently,  r...

According to The European Review for Medical and Pharmacological Sciences (2016), cells derived from the mesoderm with the ability to undergo self-renewal are mesenchymal stem cells (MSCs). These cells were first described by a team led by Friedenstein as spindle-shaped cells that adhered to the culture vessel from the bone marrow. MSCs can differentiate into several cell types such as chondrocytes (cartilage cells), osteocytes (bone cells), adipocytes (fat cells) and myocytes (muscle). 2016-published research in the Journal of Orthopaedic Surgery and Research by scientists Viganò and team explained that these cells maintain the optimum number of cells in tissues to maintain tissue homeostasis by differentiating into appropriate cells. They also release many growth factors and molecules that regulate the immune system. A team led by Sriramulu (2018) described added features of these cells in the International Journal of hematology-oncology and stem cell rese...

Consistency in research experiments is hard to achieve. There are several factors that can affect data reproducibility. Publishing data in high impact journals requires repeating experiments and standardization of techniques and procedures. We summarize 4 major factors for generating robust and reliable data. Reagents As a rule, the brand and catalog numbers of products for a specific experiment or project must be fixed to ensure low variability in the data generated. One must use reagents from reputed brands with citations to maintain credibility of the results. Verifying the quality of the reagents will save both time and effort involved in planning and execution. This can be done by a thorough literature study and checking user reviews before ordering the product. If working with cell culture , the date of opening of cell culture media and its expiry must be labeled and checked before using it. This also applies to other reagents such as FBS, trypsin or PBS, etc. Old reagents ...

In the early years of the current century, the applications associated with acute toxicity were largely being foreshadowed. However, very recently researchers have directed their attention to exploring many crucial biological pathways to evaluate underlying molecular the mechanism responsible for the diseased condition. In this regard, the  primary cells  have been emphasized to be playing a crucial role in order to facilitate high throughput testing of different drugs; and have been noted as a cheaper alternative for pre-screening drug analysis directly on humans or animals. Accordingly, a current decade has experienced an exponential increase in the rapid screening of a large number of novel drugs, to set testing parameters for safety as well as feasibility, on the basis of predictive adverse effects. Thus, the current write up is intended to promote primary cells isolated from different preliminary tissues like liver, pancreas, gingival, etc.; under strict sterile co...