Kosheeka : Primary Cells for Research

Animal use is required for preclinical investigations in life science research, which is time-consuming, costly, and causes animal cruelty. Animals being used in scientific research and tests has drawn criticism from environmentalists and animal rights activists. Several laws have also been proposed to restrict the use of animals in research. Researchers have been compelled by this to come up with alternatives that will promote compassion for animals while simultaneously reducing the number of animals exploited. In silico computer simulation and in vitro cell cultures are the two main substitutes for in vivo animal experimentation. Cell lines are now a simple tool to study nearly every subject connected to health and disease because of developments in cultural techniques. The ability to easily cultivate cells on dishes makes cell lines an effective tool for experimental studies. Cells can be easily cultivated in small containers at the workstation, and the number of cells can be simpl...

  Cancer researchers can improve and hone cell and gene therapy choices to provide individualised medical treatments through the immunomodulatory properties of mesenchymal stem cells. Cancer medicine and therapy developers can alter the functioning of the cells to promote cancer suppression by utilising mesenchymal stem cells' capacity to modify the tumour microenvironment. Mesenchymal stem cells , for instance, present a route to deliver anti-cancer medications to tumour locations due to their propensity to move to tumours. Additionally, researchers can control mesenchymal stem cells via antibody treatment and modify the angiogenesis process, which cancers depend on to thrive. Immunomodulatory effects of MSCs The immunomodulatory characteristics of MSCs offer researchers a powerful weapon against cancer, serving as the foundation for immunotherapy . The development of immune system cells such macrophages, dendritic cells, T cells, B cells, and natural killer cells (NK cells), whi...

The reproducibility and validity of scientific findings are shaped by a multitude of factors, and precise experimental methodologies, as well as the use of identical experimental substances where applicable, must be recorded. Reproducibility necessitates the characterization of reagents to assure their purity. Cell culture is an essential component of current biomedical research. Multiple quality control techniques, including cell line identification and validation that cell lines are not contaminated, are essential to assuring that reported data is reproducible. Cell line authentication is required to guarantee that cell lines have not been unintentionally incorrectly labelled or cross-contaminated, which could contribute to incorrect disease model interpretations. The necessity of quality control of cell lines used in scientific research cannot be overstated, as it is vital for data reproducibility. Two major hazards in tissue culture are: 1.        C...

  Ageing is an inescapable process that causes a steady functional impairment in the large majority of organisms. Changes in the looks of skin, which becomes thin, less supple, wrinkled, and far less able to accomplish its defensive role, are one of the most visible indicators of ageing in people. Skin ageing is a multi-faceted phenomenon, and experts are still trying to figure out all of the intricate connections that occur. We do know, however, that it is influenced by both inherent (intrinsic) and external (extrinsic) variables. Intrinsic wrinkles are characterized by skin shrinkage, an enhanced predominance of the vasculature, decreased elasticity, and fine wrinkles, and are mostly genetically driven. Extrinsic causes comprise Exposure to UV radiation and pollution, which cause intense wrinkles, rough texture, and pigmentation anomalies. A mismatch of pro-and anti-oxidants causes oxidative stress, which promotes quicker skin ageing, inflammation, and disease. Currently,  r...

According to The European Review for Medical and Pharmacological Sciences (2016), cells derived from the mesoderm with the ability to undergo self-renewal are mesenchymal stem cells (MSCs). These cells were first described by a team led by Friedenstein as spindle-shaped cells that adhered to the culture vessel from the bone marrow. MSCs can differentiate into several cell types such as chondrocytes (cartilage cells), osteocytes (bone cells), adipocytes (fat cells) and myocytes (muscle). 2016-published research in the Journal of Orthopaedic Surgery and Research by scientists Viganò and team explained that these cells maintain the optimum number of cells in tissues to maintain tissue homeostasis by differentiating into appropriate cells. They also release many growth factors and molecules that regulate the immune system. A team led by Sriramulu (2018) described added features of these cells in the International Journal of hematology-oncology and stem cell rese...

Consistency in research experiments is hard to achieve. There are several factors that can affect data reproducibility. Publishing data in high impact journals requires repeating experiments and standardization of techniques and procedures. We summarize 4 major factors for generating robust and reliable data. Reagents As a rule, the brand and catalog numbers of products for a specific experiment or project must be fixed to ensure low variability in the data generated. One must use reagents from reputed brands with citations to maintain credibility of the results. Verifying the quality of the reagents will save both time and effort involved in planning and execution. This can be done by a thorough literature study and checking user reviews before ordering the product. If working with cell culture , the date of opening of cell culture media and its expiry must be labeled and checked before using it. This also applies to other reagents such as FBS, trypsin or PBS, etc. Old reagents ...

In the early years of the current century, the applications associated with acute toxicity were largely being foreshadowed. However, very recently researchers have directed their attention to exploring many crucial biological pathways to evaluate underlying molecular the mechanism responsible for the diseased condition. In this regard, the  primary cells  have been emphasized to be playing a crucial role in order to facilitate high throughput testing of different drugs; and have been noted as a cheaper alternative for pre-screening drug analysis directly on humans or animals. Accordingly, a current decade has experienced an exponential increase in the rapid screening of a large number of novel drugs, to set testing parameters for safety as well as feasibility, on the basis of predictive adverse effects. Thus, the current write up is intended to promote primary cells isolated from different preliminary tissues like liver, pancreas, gingival, etc.; under strict sterile co...